Using the TIGR Assembler in Shotgun Sequencing Projects

TitleUsing the TIGR Assembler in Shotgun Sequencing Projects
Publication TypeBook Chapters
Year of Publication2004
AuthorsPop M, Kosack D
EditorZhao S, Stodolsky M
Book TitleBacterial Artificial Chromosomes
Series TitleMethods in Molecular Biology
Pagination279 - 294
PublisherHumana Press
ISBN Number978-1-59259-752-9

The TIGR Assembler (TA) ( 1 ) is the sequence assembly program used in sequencing projects at The Institute for Genomic Research (TIGR). Development of the TA was based on the experience obtained in more than 20 sequencing projects completed at TIGR ( see ). This extensive experience led to a sequence assembler that produces few misassemblies ( 2 , 3 ) and has been used successfully in whole-genome shotgun sequencing of prokaryotic and eukaryotic organisms, bacterial artificial chromosome-based sequencing of eukaryotic organisms, and expressed sequence tag assembly.