TY - JOUR T1 - Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract JF - Nucleic Acids ResearchNucl. Acids Res. Y1 - 1981 A1 - Dingermann,Theodor A1 - Sharp,Stephen A1 - Appel,Bernd A1 - DeFranco,Donald A1 - Mount, Stephen M. A1 - Heiermann,Reinhard A1 - Pongs,Olaf A1 - Söll,Dieter AB - We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. VL - 9 SN - 0305-1048, 1362-4962 UR - http://nar.oxfordjournals.org/content/9/16/3907 CP - 16 M3 - 10.1093/nar/9.16.3907 ER -