TY - JOUR T1 - Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non‐lethal cold shock JF - Environmental Microbiology Y1 - 2005 A1 - Boonyaratanakornkit,Boonchai B A1 - Simpson,Anjana J. A1 - Whitehead,Timothy A A1 - Fraser,Claire M. A1 - El‐Sayed, Najib M. A1 - Clark,Douglas S AB - Temperature shock of the hyperthermophilic methanarchaeon Methanococcus jannaschii from its optimal growth temperature of 85°C to 65°C and 95°C resulted in different transcriptional responses characteristic of both the direction of shock (heat or cold shock) and whether the shock was lethal. Specific outcomes of lethal heat shock to 95°C included upregulation of genes encoding chaperones, and downregulation of genes encoding subunits of the H+ transporting ATP synthase. A gene encoding an α subunit of a putative prefoldin was also upregulated, which may comprise a novel element in the protein processing pathway in M. jannaschii. Very different responses were observed upon cold shock to 65°C. These included upregulation of a gene encoding an RNA helicase and other genes involved in transcription and translation, and upregulation of genes coding for proteases and transport proteins. Also upregulated was a gene that codes for an 18 kDa FKBP-type PPIase, which may facilitate protein folding at low temperatures. Transcriptional profiling also revealed several hypothetical proteins that respond to temperature stress conditions. VL - 7 SN - 1462-2920 UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1462-2920.2005.00751.x/abstract CP - 6 M3 - 10.1111/j.1462-2920.2005.00751.x ER -