Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract
| Title | Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract |
| Publication Type | Journal Articles |
| Year of Publication | 1981 |
| Authors | Dingermann T, Sharp S, Appel B, DeFranco D, Mount SM, Heiermann R, Pongs O, Söll D |
| Journal | Nucleic Acids ResearchNucl. Acids Res. |
| Volume | 9 |
| Issue | 16 |
| Pagination | 3907 - 3918 |
| Date Published | 1981/08/25/ |
| ISBN Number | 0305-1048, 1362-4962 |
| Abstract | We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. |
| URL | http://nar.oxfordjournals.org/content/9/16/3907 |
| DOI | 10.1093/nar/9.16.3907 |