%0 Journal Article %J Nucleic Acids ResearchNucl. Acids Res. %D 1981 %T Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract %A Dingermann,Theodor %A Sharp,Stephen %A Appel,Bernd %A DeFranco,Donald %A Mount, Stephen M. %A Heiermann,Reinhard %A Pongs,Olaf %A Söll,Dieter %X We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts. %B Nucleic Acids ResearchNucl. Acids Res. %V 9 %P 3907 - 3918 %8 1981/08/25/ %@ 0305-1048, 1362-4962 %G eng %U http://nar.oxfordjournals.org/content/9/16/3907 %N 16 %R 10.1093/nar/9.16.3907