%0 Journal Article %J Microbiology and Immunology %D 2010 %T Conversion of viable but nonculturable Vibrio cholerae to the culturable state by co‐culture with eukaryotic cells %A Senoh,Mitsutoshi %A Ghosh‐Banerjee,Jayeeta %A Ramamurthy,Thandavarayan %A Hamabata,Takashi %A Kurakawa,Takashi %A Takeda,Makoto %A Rita R Colwell %A Nair,G. Balakrish %A Takeda,Yoshifumi %K conversion to culturability %K co‐culture %K eukaryotic cell %K viable but nonculturable (VBNC) Vibrio cholerae %X VBNC Vibrio cholerae O139 VC-280 obtained by incubation in 1% solution of artificial sea water IO at 4°C for 74 days converted to the culturable state when co-cultured with CHO cells. Other eukaryotic cell lines, including HT-29, Caco-2, T84, HeLa, and Intestine 407, also supported conversion of VBNC cells to the culturable state. Conversion of VBNC V. cholerae O1 N16961 and V. cholerae O139 VC-280/pG13 to the culturable state, under the same conditions, was also confirmed. When VBNC V. cholerae O139 VC-280 was incubated in 1% IO at 4°C for up to 91 days, the number of cells converted by co-culture with CHO cells declined with each additional day of incubation and after 91 days conversion was not observed. %B Microbiology and Immunology %V 54 %P 502 - 507 %8 2010/06/14/ %@ 1348-0421 %G eng %U http://onlinelibrary.wiley.com/doi/10.1111/j.1348-0421.2010.00245.x/full %N 9 %R 10.1111/j.1348-0421.2010.00245.x