TY - JOUR T1 - Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons JF - Research in Microbiology Y1 - 2013 A1 - Cantet, Franck A1 - Hervio-Heath, Dominique A1 - Caro, Audrey A1 - Le Mennec, Cécile A1 - Monteil, Caroline A1 - Quéméré, Catherine A1 - Jolivet-Gougeon, Anne A1 - Rita R Colwell A1 - Monfort, Patrick AB - Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to >1.1 × 103 MPN/l, 0.09 to 1.1 × 103 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were present in water, sediment and shellfish samples collected from these lagoons. The number of V. vulnificus in water, sediment and shellfish samples ranged from 1 to 1.1 × 103 MPN/l, 0.07 to 110 MPN/ml and 0.04 to 15 MPN/g, respectively, during summer. V. vulnificus was not detected during winter. V. cholerae was rarely detected in water and sediment during summer. In summary, results of this study highlight the finding that the three human pathogenic Vibrio spp. are present in the lagoons and constitute a potential public health hazard. VL - 164 UR - https://linkinghub.elsevier.com/retrieve/pii/S092325081300106X CP - 8 J1 - Research in Microbiology M3 - 10.1016/j.resmic.2013.06.005 ER -