TY - JOUR T1 - Solution structure and dynamics of the bioactive retroviral M domain from rous sarcoma virus JF - Journal of Molecular Biology Y1 - 1998 A1 - McDonnell,James M A1 - Fushman, David A1 - Cahill,Sean M. A1 - Zhou,Wenjun A1 - Wolven,Amy A1 - Wilson,Carol B A1 - Nelle,Timothy D A1 - Resh,Marilyn D A1 - Wills,John A1 - Cowburn,David KW - heteronuclear NMR spectroscopy KW - protein dynamics KW - RSV matrix protein KW - sequence homology KW - three-dimensional structure AB - A biologically active construct of the retroviral M domain from the avian Rous sarcoma virus is defined and its solution structure described. This M domain is fully active in budding and infectivity without myristylation. In spite of a sequence homology level that suggests no relationship among M domains and the family of matrix proteins in mammalian retroviruses, the conserved structural elements of a central core allow an M domain sequence motif to be described for all retroviruses. The surface of the M domain has a highly clustered positive patch comprised of sequentially distant residues. An analysis of the backbone dynamics, incorporating rotational anisotropy, is used to estimate the thermodynamics of proposed domain oligomerization. VL - 279 SN - 0022-2836 UR - http://www.sciencedirect.com/science/article/pii/S0022283698917880 CP - 4 M3 - 10.1006/jmbi.1998.1788 ER -