TY - JOUR T1 - Hawkeye and AMOS: Visualizing and Assessing the Quality of Genome Assemblies JF - Briefings in Bioinformatics Y1 - 2011 A1 - Schatz,Michael C A1 - Phillippy,Adam M A1 - Sommer,Daniel D A1 - Delcher,Arthur L. A1 - Puiu,Daniela A1 - Narzisi,Giuseppe A1 - Salzberg,Steven L. A1 - Pop, Mihai KW - assembly forensics KW - DNA Sequencing KW - genome assembly KW - visual analytics AB - Since its launch in 2004, the open-source AMOS project has released several innovative DNA sequence analysis applications including: Hawkeye, a visual analytics tool for inspecting the structure of genome assemblies; the Assembly Forensics and FRCurve pipelines for systematically evaluating the quality of a genome assembly; and AMOScmp, the first comparative genome assembler. These applications have been used to assemble and analyze dozens of genomes ranging in complexity from simple microbial species through mammalian genomes. Recent efforts have been focused on enhancing support for new data characteristics brought on by second- and now third-generation sequencing. This review describes the major components of AMOS in light of these challenges, with an emphasis on methods for assessing assembly quality and the visual analytics capabilities of Hawkeye. These interactive graphical aspects are essential for navigating and understanding the complexities of a genome assembly, from the overall genome structure down to individual bases. Hawkeye and AMOS are available open source at http://amos.sourceforge.net. SN - 1467-5463, 1477-4054 UR - http://bib.oxfordjournals.org/content/early/2011/12/23/bib.bbr074 M3 - 10.1093/bib/bbr074 ER -