TY - JOUR T1 - Effect of treatment on the dynamics of circulating hypodermin C in cattle naturally infested with Hypoderma lineatum (Diptera: Oestridae) JF - Veterinary Parasitology Y1 - 2003 A1 - Douglas D Colwell A1 - Panadero-Fontan,Rosario A1 - López-Sandez,Ceferino A1 - Parra-Fernandez,Francisco A1 - Paz-Silva,A A1 - Sánchez-Andrade,Rita A1 - Dı́ez-Baños,Pablo KW - Capture ELISA KW - Cattle-arthropoda KW - Control methods-arthropoda KW - Eprinomectin KW - Fenthion KW - Hypoderma spp. AB - An antigen capture ELISA, using a murine monoclonal antibody recognising recombinant hypodermin C (rHyC), was used to evaluate the influence of early treatment with eprinomectin (Eprinex®) or fenthion (Spotton®) on the kinetics of circulating hypodermin C in calves naturally infested with Hypoderma lineatum. No viable larvae were collected from treated animals, whereas a variable number of warbles were found in control animals. Treatment provoked a decrease in circulating HyC levels that was significant 9 days post-treatment (p.t.). Circulating antigen levels in the treated cattle remained detectable for approximately 99 days p.t. In contrast, control animals had no detectable antigen at 64 days p.t., 42 days earlier than in the treated animals. These results suggest that larvae were either gradually killed, resulting in slow release of antigen or they were encapsulated, leading to the slow liberation of antigen. Kinetics of circulating HyC did not differ among the two insecticide treatments. Antibodies persisted, in all groups, throughout the 120-day study. These results suggest that the antigen capture ELISA will be useful as a technique for detecting successful treatment of cattle grub infestations and for the detection of new infestations in previously infested cattle. VL - 113 SN - 0304-4017 UR - http://www.sciencedirect.com/science/article/pii/S0304401703000840 CP - 3–4 M3 - 10.1016/S0304-4017(03)00084-0 ER -