@article {19668,
	title = {Quantitative measurement of allele-specific protein expression in a diploid yeast hybrid by LC-MS},
	journal = {Molecular Systems Biology},
	volume = {8},
	year = {2012},
	month = {2012/01/01/},
	abstract = {Understanding the genetic basis of gene regulatory variation is a key goal of evolutionary and medical genetics. Regulatory variation can act in an allele-specific manner (cis-acting) or it can affect both alleles of a gene (trans-acting). Differential allele-specific expression (ASE), in which the expression of one allele differs from another in a diploid, implies the presence of cis-acting regulatory variation. While microarrays and high-throughput sequencing have enabled genome-wide measurements of transcriptional ASE, methods for measurement of protein ASE (pASE) have lagged far behind. We describe a flexible, accurate, and scalable strategy for measurement of pASE by liquid chromatography-coupled mass spectrometry (LC-MS). We apply this approach to a hybrid between the yeast species Saccharomyces cerevisiae and Saccharomyces bayanus. Our results provide the first analysis of the relative contribution of cis-acting and trans-acting regulatory differences to protein expression divergence between yeast species.Synopsis
A novel strategy for the quantitative measurement of allele-specific protein expression is used to infer the contributions of cis- and trans-acting factors influencing the divergence of protein levels between yeast species.
Rigorous experimental controls and analyses confirm the accuracy of the new strategy for the quantitative measurement of allele-specific protein expression by high-throughput mass spectrometry.Analysis of allele-specific protein expression in an interspecies yeast hybrid and protein expression differences between species reveals that both cis-effects and trans-effects contribute to protein expression divergence between two yeast species, Saccharomyces cerevisiae and Saccharomyces bayanus.
},
	keywords = {allele specific, divergence, mass spectrometry, protein expression, proteomics},
	url = {http://msb.embopress.org/content/8/1/602},
	author = {Zia Khan and Bloom, Joshua S. and Amini, Sasan and Singh, Mona and Perlman, David H. and Caudy, Amy A. and Kruglyak, Leonid}
}
